Neuron Specific Enolase, Recombinant, Human Western Blot Positive Control (NSE, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Neural Enolase, Enolase 2, ENO2)

Blue Ice

52

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

The glycolytic enzyme enolase (2-phospho-Dglycerate hydrolyase) exists as several dimeric isoenzymes (aa, ab, ag, bb and gg) composed of three distinct subunits: a, b, and g (alpha, beta, and gamma). Three isoenzymes are found in human brain: aa, ag, and gg. The ag and gg-enoloase isoenzymes are also known as neuron- specific enolase (NSE) as these isoenzymes initially were detected in neurons and neuronendocrine cells. Lung cancer is one of the most spread cancer forms with incidences about 50-100 per 100, 000 population. Approximately 20% of the lung cancer is small cell lung cancer. NSE has been shown to be a valuable tumor marker of neuroendocrine origin, particulary in small cell lung cancer and in neuroblastoma. Patients with small cell lung cancer show various proportions of ag and gg isoenzymes. The determination of NSE should detect ag and gg isoforms with the same sensitivity. The antibodies for this particular assay are specific for the g-subunit without cross reactivity with a or b subunits. NSE is reported to be useful diagnostic marker for lung cancer, neuroblastoma, melnoma, seminoma and in injury of central nervous system. In addition to the above, NSE can be a valuable tool in following-up the effect of chemotherapy of small cell lung cancer, in prognostic evaluation of patients with small cell lung cancer, and in differential diagnosis between cell lung cancer and non-small cell lung cancer.

Source:
Full-length, recombinant, human gamma-enolase (1-433aa), expressed in E. coli with N-terminal His-Tag and S-Tag sequences.

Molecular Weight:
~52kD

Applications:
Suitable for use in Western Blot. Not suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.