NRAS, Recombinant, Human (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog, N-Ras, ALPS4, GTPase NRas, NS6, HRAS1, NRAS1, OTTHUMP00000013879, OTTMUSP00000023521, Transforming Protein N-Ras, v-Ras Neuroblastoma RAS Viral Oncogene Homolog)

Blue Ice

20, 8

Supplied as a liquid in 20mM Tris HCl pH7.5, 100mM NaCl, 10% glycerol.

Neuroblastoma RAS viral (v-ras) oncogene homolog, also known as NRAS, is a member of the RAS gene family. NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Recombinant human NRAS was expressed in E. coli.

Source:
Recombinant corresponding to aa1-186 of human NRAS expressed in E. coli.

AA Sequence:
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI KRVKDSDDVP MVLVGNKCDL PTRTVDTKQA HELAKSYGIP FIETSAKTRQ GVEDAFYTLV REIRQYRMKK LNSSDDGTQG
CMGLPC

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

NRAS, Recombinant, Human (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog, N-Ras, ALPS4, GTPase NRas, NS6, HRAS1, NRAS1, OTTHUMP00000013879, OTTMUSP00000023521, Transforming Protein N-Ras, v-Ras Neuroblastoma RAS Viral Oncogene Homolog)