PCMT1, aa1-227, Recombinant, Human (L-isoaspartyl Protein Carboxyl Methyltransferase, PIMT, Protein-beta-aspartate Methyltransferase, Protein-L-isoaspartate(D-aspartate) O-methyltransferase, Protein L-isoaspartyl/D-aspartyl Methyltransferase)

Blue Ice

28, 8

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol, 0.1M sodium chloride.

PCMT1, also known as L-isoaspartyl protein carboxyl methyltransferase, is an enzyme that catalyses the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. This protein plays a role in the repair and/or degradation of damaged proteins.

Source:
Recombinant corresponding to aa1-227 of human PCMT1 6x his tagged, expressed in E.coli.

AA Sequences:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAWK SGGASHSELI HNLRKNGIIK TDKVFEVMLA TDRSHYAKCN PYMDSPQSIG FQATISAPHM HAYALELLFD QLHEGAKALD GSGSGILTA CFARMVGCTG KVIGIDHIKE LVDDSINNVR KDDPTLLSSG RVQLVVGDGR MGYAEEAPYD AIHVGAAAPV VPQALIDQLK PGGRLILPVG PAGGNQMLEQ YDKLQDGSIK MKPLMGVIYV PLTDKEKQWS RWK

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

PCMT1, aa1-227, Recombinant, Human (L-isoaspartyl Protein Carboxyl Methyltransferase, PIMT, Protein-beta-aspartate Methyltransferase, Protein-L-isoaspartate(D-aspartate) O-methyltransferase, Protein L-isoaspartyl/D-aspartyl Methyltransferase)