PRMT1, isoform2, Recombinant, Mouse, 1-353 aa, mouse, His-MBP tag (Protein arginine N Methyltransferase 1)

Blue Ice

84

Supplied as a liquid in 40mM Tris, pH 8.0, 100mM sodium chloride, 4mM MgCl2, 2mM DTT, 40% glycerol.

Murine PRMT1 isoform2, 1-353 aa, His-MBP tagged, Recombinant, E. coli

Full name: protein arginine N-methyltransferase 1

NCBI Accession #: AAF37293

Synonyms: Mrmt1, Hrmt112, AW214366, Protein arginine N-methytransferase 1

Protein arginine N-methyltransferase 1(PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins.

Mouse PRMT1(AAF37293) shares 99.9% sequence identity with the human proteins (NP_938074).

Recombinant mouse PRMT1, fused to His-MBP tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 84kD (750 aa)

Specific Activity: 10, 000unit/ml

Unit Definition: One unit will transfer 1pmol of methyl group to synthetic peptide of histone H4 for 10 minutes at 37℃.

Sequence: MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE
NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW
SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA
VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS
GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK
PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI
LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS
EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG
FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV
HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA
KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.