SIV gp130, mac251, Recombinant (Simian Immunodeficiency Virus)

Molecular Biology / MB-Infectious Disease

-70°C

Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.

Full length Recombinant SIV gp130 glycoprotein produced in the Baculovirus Expression System.

Specificity:
This protein binds to murine monoclonal antibodies of defined epitope specificity and simian serum polyclonal antibodies in ELISA and Western Blot.

Biological Activity:
Binds to human T-cell receptor CD4 in ELISA and Western Blot as determined by CD4/gp130/Anti-gp130 mAb-peroxidase capture ELISA. Activates simian T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.

Applications:
Suitable for use in CD4 Binding, B and T cell Activation, ELISA and Western Blot. Also suitable for immunization. Other applications not tested.

Recommended Dilutions:
ELISA: 10-100ng
Western Blot: 10-100ng
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.