SNAP 25, Recombinant, Human, His-Tag, aa1-206 (SNAP25, Synaptosomal-associated Protein 25)

Blue Ice

25, 4

Supplied as a liquid in 50mM HEPES pH 7.4, 100mM sodium chloride, 5mM DTT.

The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters. Recombinant human SNAP25 was purified using conventional chromatography techniques.

Source:
Recombinant protein corresponding to aa1-206 from human SNAP25, fused to His-Tag at N-terminus, expressed in E. coli.

Molecular Weight:
25.4kD (226 amino acids)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK
DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL
RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.