SPHK2, Long Form, Recombinant, Human (Sphingosine kinase 2, SK 2)

Dry Ice

74

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween 20, 50% glycerol and 3mM DTT.

Human Sphingosine kinase 2 ( GenBank Accession No.NM_020126), full length with N-terminal His tag, expressed in a Baculovirus infected Sf9 cell expression system.

Specific Activity:
20 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min.

Assay Buffer:
50mM HEPES, pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 4uM D-erythrosphingosine, and 0.75ug/ml sphingosine kinase 2.

Applications:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.