5?-Nucleotidase Assay Kit

5?-Nucleotidase Assay Kit is an Assay Kit by Enzymatic Colorimetric Method.

Based on the bottle type, product model can be classified into Hitachi 7170, Hitachi 7060, Hitachi 7020, Beckman, Toshiba, Mindray, DuPont, Innova, Siemens, KHB, Abbott, Universal types, etc.

This product is used to determine 5?-Nucleotidase activity in human serum.

The kit is based on continuous monitoring assay use the 5?-Nucleotidase coupling purine nucleoside phosphorylase (PNP), xanthine oxidase (XOD) and peroxidase (POD). 5'-nucleotidase hydrolyze inosine acid to generate inosine, then coupled by the action of PNP to generate hypoxanthine. Uric Acid and hydrogen peroxide formed from hypoxanthine under Oxidation of XOD. Finally, Fuchsia colored quinone was produced under POD effect by the Trinder reaction. The 5'-nucleotidase activity can be calculated by measuring the increase rate of colored quinone absorbance at 550nm wavelength.

This assay kit is suitable for automatic or semi-automatic biochemical analyzer with 546nm and 800nm wavelength.

Serum without hemolysis used as sample. Samples testing should be completed at the same day after collected. Otherwise, the samples should be cryopreserved and avoid repeated freeze-thaw cycles. 5?-Nucleotidase in the samples remains stable for 7days at 2-8C and for 3 months at cryopreservation condition.
This kit comes with a calibrator and a control.

Not applicable.

5?-Nucleotidase