Creatine Kinase Isoenzyme MB Assay Kit

Creatine Kinase Isoenzyme MB Assay Kit is an Assay Kit by Selective Inhibition Method.

Based on the bottle type, product model can be classified into Hitachi 7170, Hitachi 7060, Hitachi 7020, Beckman, Toshiba, Mindray, DuPont, Innova, Siemens, KHB, Abbott, Universal types, etc.

This product is used to determine Creatine Kinase Isoenzyme MB (CK - MB) activity in human serum.

Creatine kinase isozyme is mainly divided into CK - MB, CK - BB, and CK - MM. There is nearly no CKBB in normal serum. So the anti - serum special to M - subunit could form antigen - antibody complex with M - subunit of CKMM and CKMB. The reaction completely inhibit the activity of M - subunit. The activity of CK - MB is equivalent to activity of CK Multiplied by 2. CK catalyzes the reaction between phosphocreatine and Adenosine diphosphate (ADP) to create adenosine triphosphate (ATP) and Creatine. Then Glucose - 6 - phosphate ge nerated from glucose and ATP un der Hexokinase (HK) effect. Final, nicotinamide adenine dinucleotide phosphate (NADPH) and 6 - phosphoric acid glucose ester formed from Glucose - 6 - phosphate under the oxidation of Glucose - 6 - phosphate dehydrogenase (G6PDH). The generated rate of NADPH stand s for the activity of CK.

This assay kit is suitable for automatic or semi - automatic biochemical analyzer with 340nm and 405nm wavelength.

Use fasting serum. Samples testing should be completed at the same day after collected. Otherwise, the samples should be cryopreserved and avoid repeated freeze - thaw cycles. Creatine Kinase Isoenzyme MB in the sa mples remain s stable for 2 days at 2 - 8C and for 3 months at cryopreservation condition.

Not applicable.

Creatine Kinase Isoenzyme MB