RIN-14B cells

Disease:
Islet cell tumor, rat insulinoma; these cells offer models for the study of the biology of pancreatic islet cells, specifically the mechanisms controlling the synthesis, storage and secretion of insulin and somatostatin.
Origin:
The RIN-14B cell line is a secondary clone derived from the RIN-m rat islet line.
Species:
Rattus norvegicus, rat
Tissue:
Pancreas, islet cell tumor
Morphology:
Epithelial-like
Properties:
Adherent
Strain:
Male, NEDH.
Medium:
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: RIN-14B (AddexBio C0018020)