RIN-14B cells

Disease:

Islet cell tumor, rat insulinoma; these cells offer models for the study of the biology of pancreatic islet cells, specifically the mechanisms controlling the synthesis, storage and secretion of insulin and somatostatin.

Origin:

The RIN-14B cell line is a secondary clone derived from the RIN-m rat islet line.

Species:

Rattus norvegicus, rat

Tissue:

Pancreas, islet cell tumor

Morphology:

Epithelial-like

Properties:

Adherent

Strain:

Male, NEDH.

Medium:

AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO