SGK1, 60-431 aa, Human, His tag, HI-5

SGK1, also known as serum-and glucocorticoid-regulated kinase, is a serine/threonine protein kinase and a member of the ''AGC'' subfamily, which includes protein kinases A, G, and C. This protein plays an important role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. It is activated in vitroby 3-phosphoinositide-dependent protein kinase-1 (PDK-1) and in vivoin response to signals that activate phosphatidylinositol (PI) 3-kinase. Recombinant human SGK1 protein was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.25 mg/ml (determined by Bradford assay)

• Maiyar A.C. et al. (1996) J. Biol. Chem.. 271: 12414-12422.
• Brunet A. et al. (2001) Mol. Cell. Biol. 21:952-65

>, 90% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MISQPQEPEL MNANPSPPPS PSQQINLGPS SNPHAKPSDF HFLKVIGKGS FGKVLLARHK AEEVFYAVKV LQKKAILKKK EEKHIMSERN VLLKNVKHPF LVGLHFSFQT ADKLYFVLDY INGGELFYHL QRERCFLEPR ARFYAAEIAS ALGYLHSLNI VYRDLKPENI LLDSQGHIVL TDFGLCKENI EHNSTTSTFC GTPEYLAPEV LHKQPYDRTV DWWCLGAVLY EMLYGLPPFY SRNTAEMYDN ILNKPLQLKP NITNSARHLL EGLLQKDRTK RLGAKDDFME IKSHVFFSLI NWDDLINKKI TPPFNPNVSG PNDLRHFDPE FTEEPVPNSI GKSPDSVLVT ASVKEAAEAF LGFSYAPPTD SFL