SNRPA1, 1-255aa , Human, His tag E.coli

SNRPA1, also known as U2 small nuclear ribonucleoprotein A, is a component of the U2 snRNP that forms a complex with U2 snRNP B (U2B). Together, U2 snRNP A and U2 snRNP B form a complex that binds to the U2 snRNA hairpin IV. The configuration of this U2 snRNP A/U2 snRNP B dimer and the subtle variations of a few key residues regulate the snRNP-RNA-binding specificity. U2 snRNP A is a 255 amino acid protein, and two isoforms exist as a result of alternative splicing events. Recombinant human SNRPA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.5 mg/ml (determined by Bradford assay)

• Crispino J.D. et al. (1994) Science 265: 1866-1869.
• Price S.R. et al. (1998) Nature. 394: 645-650.

>, 80% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPCL TELILTNNSL VELGDLDPLA SLKSLTYLSI LRNPVTNKKH YRLYVIYKVP QVRVLDFQKV KLKERQEAEK MFKGKRGAQL AKDIARRSKT FNPGAGLPTD KKKGGPSPGD VEAIKNAIAN ASTLAEVERL KGLLQSGQIP GRERRSGPTD DGEEEMEEDT VTNGS