LSM2, 1-95aa , Human, His tag, E.coli

LSM2 (U6 snRNA-associated Sm-like protein LSm2) belongs to the snRNP Sm proteins family. Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. LSM2 binds specifically to the 3'-terminal U-tract of U6 snRNA. It is involved in pre-mRNA splicing. Recombinant human LSM2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.5mg/ml (determined by Bradford assay)

• Lehner, Ben, et al. (2004) Genome Res. (United States) 14 (7): 1315-23.
• Achsel T., et al. (1999) EMBO J. 18:5789-5802

>, 90% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MGSHMLFYSF FKSLVGKDVV VELKNDLSIC GTLHSVDQYL NIKLTDISVT DPEKYPHMLS VKNCFIRGSV VRYVQLPADE VDTQLLQDAA RKEALQQKQ