Granulocyte Colony-Stimulating Factor, G-CSF, Mouse, Recombinant

G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant murine G-CSF is a 19.0 kDa protein consisting of 179 amino acid residues.

CSF-3, MGI-1G, GM-CSFb, pluripoietin

E. Coli

MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA

The ED 50 was determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is a‰¤ 0.05 ng/ml, corresponding to a specific activity of a‰¥ 2 x 10^7 units/mg.

Verified by N-terminal and Mass Spectrometry analyses (when applicable).