Goat anti-BIF-1 / SH3GLB1 Antibody

Pep ELISA, WB, IHC

Goat anti-BIF-1 / SH3GLB1 Antibody
Principle Name: BIF-1 / SH3GLB1
Official Symbol: SH3GLB1
All Name and Symbols: SH3GLB1; Bif-1; SH3-domain GRB2-like endophilin B1; CGI-61; KIAA0491; endophilin B1; Bax-interacting factor 1; SH3-containing protein SH3GLB1; SH3-domain, GRB2-like, endophilin B1; SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1; dJ612B15.2
Accession Number(s): NP_057093.1
Peptide Sequence: QKGKVPITYLELLN, is from C Terminus
Human Gene ID(s): 51100
Non-Human GeneID(s): 54673 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 38kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 ug/ml. A minor band of unknown identity was also con
Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 2.5ug/ml.
Reference(s): Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti SM, Youle RJ, Wang HG. Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis. Mol Cell Biol. 2005 Nov; 25(21):9369-82.PMID: 16227588