Goat anti-LRP6 Antibody

Pep ELISA, IHC

Goat anti-LRP6 Antibody
Principle Name: LRP6
Official Symbol: LRP6
All Name and Symbols: LRP6; low density lipoprotein receptor-related protein 6; low density lipoprotein-related protein 6; ADCAD2; FLJ90062; FLJ90421
Accession Number(s): NP_002327.2
Peptide Sequence: CMTSVATAKGYTSDL, is from internal region
Human Gene ID(s): 4040
Non-Human GeneID(s): 16974 (mouse);
Important Comments: This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Preliminary experiments gave bands at approx 50kDa and 35kDa in Human Adipose lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated
Immunohistochemistry: In paraffin embedded Human Kidney shows luminar membrane staining in DCT. Recommended concentration: 4-6ug/ml.
Reference(s): Tamai K, Semenov M, Kato Y, Spokony R, Liu C, Katsuyama Y, Hess F, Saint-Jeannet JP, He X. LDL-receptor-related proteins in Wnt signal transduction. Nature. 2000 Sep 28; 407(6803):530-5. .PMID: 11029007