Goat anti-DIO2 Antibody

Pep ELISA, IHC

Goat anti-DIO2 Antibody
Principle Name: DIO2
Official Symbol: DIO2
All Name and Symbols: DIO2; deiodinase, iodothyronine, type II ; 5DII; D2; SelY; TXDI2 ; thyroxine deiodinase, type II; type 2 iodothyronine deiodinase; type II iodothyronine deiodinaseN: type-II 5'deiodinase
Accession Number(s): NP_000784.2; NP_054644.1; NP_001007024.1
Peptide Sequence: EVKKHQNQEDRC, is from internal region
Human Gene ID(s): 1734
Non-Human GeneID(s): 13371 (mouse); 65162 (rat);
Important Comments: This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments gave an approx 65-70kDa band in Human Heart and Thyroid lysates after 0.05ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculat
Immunohistochemistry: Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration: 2-3ug/ml. Data obtained from customer.
Reference(s): Gumieniak O, Perlstein TS, Williams JS, Hopkins PN, Brown NJ, Raby BA, Williams GH. Ala92 Type 2 Deiodinase Allele Increases Risk for the Development of Hypertension. Hypertension. 2007 Jan 15; [Epub ahead of print] .PMID: 17224473