Goat anti-LASP1 (internal region) Antibody

Pep ELISA, WB, IHC

Goat anti-LASP1 (internal region) Antibody
Principle Name: LASP1 (internal region)
Official Symbol: LASP1
All Name and Symbols: LASP1; LIM and SH3 protein 1; Lasp-1; MLN50
Accession Number(s): NP_006139.1
Peptide Sequence: PERRDSQDGSSYR, is from internal region
Human Gene ID(s): 3927
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 36kDa band observed in Human Kidney lysates (calculated MW of 29.7kDa according to NP_006139.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreiber et al, Gene. 1998 Jan
Immunohistochemistry: Paraffin embedded Human Colon and Small Intestine. Recommended concentration: 2.5ug/ml.
Reference(s): Grunewald TG, Kammerer U, Schulze E, Schindler D, Honig A, Zimmer M, Butt E. Silencing of LASP-1 influences zyxin localization, inhibits proliferation and reduces migration in breast cancer cells. Exp Cell Res. 2006 Apr 15; 312(7):974-82. Epub 2006 Jan 23. Erratum in: Exp Cell.PMID: 16430883