Goat anti-CHRNB4 Antibody

Pep ELISA, IHC

Goat anti-CHRNB4 Antibody
Principle Name: CHRNB4
Official Symbol: CHRNB4
All Name and Symbols: CHRNB4 ; cholinergic receptor; nicotinicN: beta 4 ; Cholinergic receptor, neuronal nicotinic, beta polypeptide-4; cholinergic receptor, nicotinic, beta polypeptide 4; neuronal nicotinic receptor beta 4 subunit
Accession Number(s): NP_000741.1
Peptide Sequence: DYRLTWNSSRYEGVN, is from internal region
Human Gene ID(s): 1143
Important Comments: The immunizing peptide represents part of the first extracellular domain (aa22-236).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4
Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 2.5ug/ml.
Reference(s): Liang Y, Salas R, Marubio L, Bercovich D, De Biasi M, Beaudet AL, Dani JA. Functional polymorphisms in the human beta4 subunit of nicotinic acetylcholine receptors. Neurogenetics. 2005 Feb; 6(1):37-44. Epub 2004 Nov 25.PMID: 15742216