Goat anti-CHRNA4 (aa29-43) Antibody

Pep ELISA, WB

Goat anti-CHRNA4 (aa29-43) Antibody
Principle Name: CHRNA4 (aa29-43)
Official Symbol: CHRNA4
All Name and Symbols: CHRNA4; cholinergic receptor, nicotinic, alpha 4; BFNC; EBN; EBN1; NACRA4; cholinergic receptor, nicotinic, alpha 4 subunit; cholinergic receptor, nicotinic, alpha polypeptide 4; neuronal acetylcholine receptor alpha 4 subunit; neuronal nicotinic acetylcho
Accession Number(s): NP_000735.1
Peptide Sequence: HVETRAHAEERLLKK, is from internal region
Human Gene ID(s): 1137
Non-Human GeneID(s): 11438 (mouse); 25590 (rat);
Important Comments: This antibody is expected NOT to cross-react with the similar alpha 2 subunit.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consi
Reference(s): Schwarz J, Schwarz SC, Dorigo O, Stützer A, Wegner F, Labarca C, Deshpande P, Gil JS, Berk AJ, Lester HA. Enhanced expression of hypersensitive alpha4* nAChR in adult mice increases the loss of midbrain dopaminergic neurons. FASEB J. 2006 May; 20(7):935-46.PMID: 16675851