Goat anti-CENTG1 / PIKE Antibody

Pep ELISA, WB, IHC

Goat anti-CENTG1 / PIKE Antibody
Principle Name: CENTG1 / PIKE
Official Symbol: AGAP2
All Name and Symbols: CENTG1; centaurin, gamma 1; AGAP2; FLJ16430; GGAP2; KIAA0167; PIKE; Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2; GTP-binding and GTPase activating protein 2; centaurin gamma1; phosphoinositide 3-kinase enhancer
Accession Number(s): NP_055585.1
Peptide Sequence: QASLDSIREAVINSQ, is from internal region
Human Gene ID(s): 116986
Important Comments: This antibody is expected to recognize isoform PIKE-S (NP_055585.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 100kDa band observed in Human Brain (Amygdala and Cerebral Cortex) lysates (calculated MW of 90.5kDa according to NP_055585.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: Paraffin embedded Human Thyroid and Kidney. Recommended concentration: 3.75ug/ml.
Reference(s): Liu Z, Jang SW, Liu X, Cheng D, Peng J, Yepes M, Li XJ, Matthews S, Watts C, Asano M, Hara-Nishimura I, Luo HR, Ye K. Neuroprotective actions of PIKE-L by inhibition of SET proteolytic degradation by asparagine endopeptidase. Mol. Cell 2008 Mar 29 (6): 665-78.PMID: 18374643