Goat anti-F2R / PAR1 Antibody

Pep ELISA, WB, IHC

Goat anti-F2R / PAR1 Antibody
Principle Name: F2R / PAR1
Official Symbol: F2R
All Name and Symbols: F2R; coagulation factor II (thrombin) receptor; CF2R; HTR; PAR1; TR; coagulation factor II receptor; protease-activated receptor 1; thrombin receptor
Accession Number(s): NP_001983.2
Peptide Sequence: TRARRPESKATNAT, is from Internal region (near N Terminus)
Human Gene ID(s): 2149
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 48kDa band observed in lysates of cell line HeLa (calculated MW of 47.4kDa according to NP_001983.2). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 2.5ug/ml.
Reference(s): Niessen F, Schaffner F, Furlan-Freguia C, Pawlinski R, Bhattacharjee G, Chun J, Derian CK, Andrade-Gordon P, Rosen H, Ruf W. Dendritic cell PAR1-S1P3 signalling couples coagulation and inflammation. Nature. 2008 Apr 3; 452(7187):654-8. Epub 2008 Feb 27.PMID: 18305483