Goat anti-CHRNA7 Antibody

Pep ELISA, IHC

Goat anti-CHRNA7 Antibody
Principle Name: CHRNA7
Official Symbol: CHRNA7
All Name and Symbols: CHRNA7; cholinergic receptor, nicotinic, alpha 7; NACHRA7; a7 nicotinic acetylcholine receptor; alpha 7 neuronal nicotinic acetylcholine receptor; alpha-7 nicotinic cholinergic receptor subunit; alpha7 nicotinic receptor subunit; cholinergic receptor, nic
Accession Number(s): NP_000737.1
Peptide Sequence: KRPGEDKVRPACQHKQ, is from internal region
Human Gene ID(s): 1139
Non-Human GeneID(s): 11441 (mouse); 25302 (rat);
Important Comments: This antibody also reacts with CHRNA7-FAM7A fusion isoform 1 (NP_647536.1) and isoform 2 (NP_683709.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Immunohistochemistry: In paraffin embedded Rat Cerebral Cortex and Rat Hippocampus shows membranous staining in neural cells. Recommended concentration, 5-10ug/ml.
Reference(s): Ren K, King MA, Liu J, Siemann J, Altman M, Meyers C, Hughes JA, Meyer EM The alpha7 nicotinic receptor agonist 4OH-GTS-21 protects axotomized septohippocampal cholinergic neurons in wild type but not amyloid-overexpressing transgenic mice Neuroscience. 2007 Aug 10; 148(1):230-7. Epub 2007 Jul 20.PMID: 17640819