Goat anti-MYO5A Antibody

Goat anti-MYO5A Antibody
Principle Name: MYO5A
Official Symbol: MYO5A
All Name and Symbols: MYO5A; myosin VA (heavy chain 12, myoxin); GS1; MYH12; MYO5; MYR12; dilute; myosin V; myosin VA; myosin, heavy polypeptide kinase; myoxin
Accession Number(s): NP_000250.3; NP_001135967.1
Peptide Sequence: ETKQLELDLN, is from internal region
Human Gene ID(s): 4644
Non-Human GeneID(s): 17918 (mouse); 25017 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IF
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3). Recommended concentration: 1-3ug/ml.
Reference(s): Guey LT, García-Closas M, Murta-Nascimento C, Lloreta J, Palencia L, Kogevinas M, Rothman N, Vellalta G, Calle ML, Marenne G, Tardón A, Carrato A, García-Closas R, Serra C, Silverman DT, Chanock S, Real FX, Malats N, Genetic susceptibility to distinct bladder cancer subphenotypes. European urology 2010 Feb 57 (2): 283-92.PMID: 19692168