Goat anti-IRF2BP1 (aa109-122) Antibody

Pep ELISA, WB

Goat anti-IRF2BP1 (aa109-122) Antibody
Principle Name: IRF2BP1 (aa109-122)
Official Symbol: IRF2BP1
All Name and Symbols: IRF2BP1; interferon regulatory factor 2 binding protein 1; DKFZp434M154
Accession Number(s): NP_056464.1
Peptide Sequence: QDRYDRATSSGRLP, is from internal region
Human Gene ID(s): 26145
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 75kDa band observed in lysates of cell line HepG2 and 80kDa in nuclear lysates of cell line NIH3T3 (calculated MW of 61.7kDa according to NP_056464.1). Recommended concentration: 0.1-0.3ug/ml.
Reference(s): Kimura M, IRF2-binding protein-1 is a JDP2 ubiquitin ligase and an inhibitor of ATF2-dependent transcription. FEBS letters 2008 Aug 582 (19): 2833-7.PMID: 18671972