Goat anti-HSPC117 (aa201-215) Antibody

Pep ELISA, WB, IHC

Goat anti-HSPC117 (aa201-215) Antibody
Principle Name: HSPC117 (aa201-215)
Official Symbol: C22orf28
All Name and Symbols: C22orf28; chromosome 22 open reading frame 28; DJ149A16.6; RP1-149A16.6; hypothetical protein LOC51493; novel protein HSPC117
Accession Number(s): NP_055121.1
Peptide Sequence: QADPNKVSARAKKR, is from internal region
Human Gene ID(s): 51493
Non-Human GeneID(s): 28088 (mouse); 362855 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 55kDa band observed in lysates of cell lines HeLa, Jurkat and K562 (calculated MW of 55.2kDa according to NP_055121.1). Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry: In paraffin embedded Human Testis shows nuclear and vicinity staining in spermatogonia. Recommended concentration, 5-10ug/ml.
Reference(s): Popow J, Englert M, Weitzer S, Schleiffer A, Mierzwa B, Mechtler K, Trowitzsch S, Will CL, Lührmann R, Söll D, Martinez J. HSPC117 is the essential subunit of a human tRNA splicing ligase complex. Science. 2011 Feb 11; 331(6018):760-4.PMID: 21311021