Goat anti-PLEKHA5 / PEPP2 Antibody

Goat anti-PLEKHA5 / PEPP2 Antibody
Principle Name: PLEKHA5 / PEPP2
Official Symbol: PLEKHA5
All Name and Symbols: PLEKHA5; pleckstrin homology domain containing, family A member 5; FLJ10667; FLJ26734; FLJ31492; KIAA1686; PEPP-2; PEPP2; OTTHUMP00000237298; OTTHUMP00000237299; OTTHUMP00000237300; OTTHUMP00000237302; OTTHUMP00000239732; PH domain-containing family A mem
Accession Number(s): NP_061885.2; NP_001137293.2
Peptide Sequence: RENDVKPDHETP, is from internal region
Human Gene ID(s): 54477
Important Comments: This antibody is expected to recognize isoform 1 (NP_061885.2) and isoform 2 (NP_001137293.2) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 2000.
Western Blot: Preliminary experiments in lysates of Human Testis, HeLa and MOLT4 gave no specific signal but low background (at antibody concentration up to 2ug/ml). We would appreciate any feedback from people in the field - have any results been report
Reference(s): Dowler S, Currie RA, Campbell DG, Deak M, Kular G, Downes CP, Alessi DR. Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities. Biochem J. 2000 Oct 1; 351(Pt 1):19-31.PMID: 11001876