Goat anti-NDUFA7 Antibody

Pep ELISA, WB, IHC

Goat anti-NDUFA7 Antibody
Principle Name: NDUFA7
Official Symbol: NDUFA7
All Name and Symbols: NDUFA7; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa; B14.5a; CI-B14.5a; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7; NADH-ubiquinone oxidoreductase subunit B14.5a; complex I B14.5a subunit
Accession Number(s): NP_004992.2
Peptide Sequence: PKLPVGPSHKLSNN, is from internal region
Human Gene ID(s): 4701
Non-Human GeneID(s): 66416 (mouse); 299643 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 15kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 12.6kDa according to NP_004992.2). Recommended concentration: 0.03-0.1ug/ml.
Immunohistochemistry: In paraffin embedded Human Heart shows mitochondrial staining in cardial fibres. Recommended concentration, 4-8ug/ml.
Reference(s): Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. PLoS One. 2010 Sep 21; 5(9):e12862.PMID: 20877624