Goat anti-Sp1(mouse) Antibody

Pep ELISA, WB

Goat anti-Sp1(mouse) Antibody
Principle Name: Sp1(mouse)
Official Symbol: Sp1
All Name and Symbols: Sp1; trans-acting transcription factor 1; 1110003E12Rik; AA450830; AI845540; Sp1-1
Accession Number(s): NP_038700.2
Peptide Sequence: DGIHDETAGGEGEN, is from internal region
Non-Human GeneID(s): 20683 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 100kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 80.4kDa according to NP_038700.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. R
Reference(s): Uren AG, Kool J, Matentzoglu K, de Ridder J, Mattison J, van Uitert M, Lagcher W, Sie D, Tanger E, Cox T, Reinders M, Hubbard TJ, Rogers J, Jonkers J, Wessels L, Adams DJ, van Lohuizen M, Berns A, Large-scale mutagenesis in p19(ARF)- and p53-deficient mice identifies cancer genes and their collaborative networks. Cell 2008 May 133 (4): 727-41.PMID: 18485879