Interleukin-15 Human Recombinant

Lyophilized powder at room temperature

Interleukin-15 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids (and an N-terminal Methionine) and having a molecular mass of 12.8kDa. The IL-15 is purified by proprietary chromatographic techniques.
Amino Acid Sequence:MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS.
Physical Appearance and Stability: Lyophilized Interleukin-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL15 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The protein was lyophilized from PBS pH 7.4. Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-15 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 was found to be < 0.5 ng/ml, corresponding to a Specific Activity of 2 x 106 IU/mg.