Staphylococcal Protein-A Cys Recombinant

Lyophilized powder at room temperature.

SPA-Cys Recombinant produced in E.Coli is a single non-glycosylated polypeptide chain with a Cys on C-terminus. SPA-Cys is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 297 amino acids and having a molecular mass of 33.5kDa containing little or no carbohydrate. Cell wall binding region, cell membrane binding region and albumin binding region were removed to ensure the highest specific IgG binding.
Amino Acid Sequence:NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDC
Physical Appearance and Stability: Lyophilized SPA although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution SPA should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity: SPA protein was lyophilized with no additives. Greater than 98.0% as determined by:
(a) Analysis by HPLC.
(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized SPA in sterile 18MO-cm H2O not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions.