RAD51B Human Recombinant

Lyophilized powder at room temperature

RAD51B Human Recombinant produced in E. coli is. a single polypeptide chain containing 373 amino acids (1-350) and having a molecular mass of 40.6kDa. RAD51B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMGSKKLK RVGLSQELCD RLSRHQILTC QDFLCLSPLE LMKVTGLSYR GVHELLCMVS RACAPKMQTA YGIKAQRSAD FSPAFLSTTL SALDEALHGG VACGSLTEIT GPPGCGKTQF CIMMSILATL PTNMGGLEGA VVYIDTESAF SAERLVEIAE SRFPRYFNTE EKLLLTSSKV HLYRELTCDE VLQRIESLEE EIISKGIKLV ILDSVASVVR KEFDAQLQGN LKERNKFLAR EASSLKYLAE EFSIPVILTN QITTHLSGAL ASQADLVSPA DDLSLSEGTS GSSCVIAALG NTWSHSVNTR LILQYLDSER RQILIAKSPL APFTSFVYTI KEEGLVLQAY GNS.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: The RAD51B solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea. Greater than 85% as determined by SDS-PAGE.