Vergleich

Anti-Human Eotaxin-2 Antibody

ArtNr ABC-ABG10091-U500
Hersteller Abcepta
Menge 500 ug
Kategorie
Typ Antibody Primary
Applikationen WB, IHC, ELISA
Specific against other
Host Mouse
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Application
WB, IHC, E
Clonality
Monoclonal
Reactivity
H
Preparation
Produced in BALB/c mice using highly pure (> 98%) recombinant human Eotaxin-2 as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by antigen affinity chromatography.
WesternBlot
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µ g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
Sandwich
In a sandwich ELISA (assuming 100µ l/well), a concentration of 2.0-4.0 µ g/ml of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with BioGems' biotinylated antigen affinity purified anti-human Eotaxin-2 (60-123BT) as the detection antibody at a concentration of approximately 1.0-2.0 µ g/ml.
Immunohistochemistry
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 μ g/ml with an overnight incubation at 4?C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
Formulation
A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 500 ug
Lieferbar: In stock
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