CDH10 Antibody (C-term)

Synthetic Peptide

CDH10 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is predominantly expressed in brain and is putatively involved in synaptic adhesions, axon outgrowth and guidance.

Polyclonal

1008

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-CDH10 Antibody (C-term) at 1:2000 dilution
Lane 1: SK-BR-3 whole cell lysate
Lane 2: NCI-H460 whole cell lysate
Lane 3: F9 whole cell lysate


Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 88 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH10 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

1:10~50

FC

P70408; P79995

Rabbit Ig

495-523

This CDH10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-523 amino acids from the C-terminal region of human CDH10.