Vergleich

COXIV Antibody

ArtNr ABC-AP22111a
Hersteller Abcepta
Menge 200 ul
Kategorie
Typ Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen WB, FC
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products mitochondrial, Cox4i1, Cytochrome c oxidase subunit 4 isoform 1, COX IV-1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, Cox4, Cox4a
Lieferbar
Primary Accession
P19783
Antigen Type
Synthesized Peptide
Application
WB, FC
Bio Background
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Bio References
Grossman L.I., et al.Nucleic Acids Res. 18:6454-6454(1990).
Carter R.S., et al.Arch. Biochem. Biophys. 288:97-106(1991).
Carninci P., et al.Science 309:1559-1563(2005).
Lubec G., et al.Submitted (APR-2007) to UniProtKB.
Park J., et al.Mol. Cell 50:919-930(2013).
Clonality
polyclonal
Clone/Animal Names
RB55992
Gene ID
12857
Gene Name
Cox4i1
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H, M, Rat
Legend image 1
All lanes : Anti-COXIV Antibody at 1:2000 dilution
Lane 1: NIH/3T3 whole cell lysate
Lane 2: BA/F3 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: C6 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 20 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1
WB
Dilution image 1
1:2000
Legend image 2
Overlay histogram showing NIH/3T3 cells stained with AP22111a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22111a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 2
FC
Dilution image 2
1:25
Other Accession
P10888
Predicted
Rat
Isotype
Rabbit Ig
Calculated MW
19530
Antigen Source
HUMAN
Target/Specificity
This COXIV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 115-169 amino acids from the mouse region of human COXIV.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 200 ul
Lieferbar: In stock
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