Comparison

COXIV Antibody

Item no. ABC-AP22111a
Manufacturer Abcepta
Amount 200 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications WB, FC
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products mitochondrial, Cox4i1, Cytochrome c oxidase subunit 4 isoform 1, COX IV-1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, Cox4, Cox4a
Available
Primary Accession
P19783
Antigen Type
Synthesized Peptide
Application
WB, FC
Bio Background
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Bio References
Grossman L.I., et al.Nucleic Acids Res. 18:6454-6454(1990).
Carter R.S., et al.Arch. Biochem. Biophys. 288:97-106(1991).
Carninci P., et al.Science 309:1559-1563(2005).
Lubec G., et al.Submitted (APR-2007) to UniProtKB.
Park J., et al.Mol. Cell 50:919-930(2013).
Clonality
polyclonal
Clone/Animal Names
RB55992
Gene ID
12857
Gene Name
Cox4i1
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H, M, Rat
Legend image 1
All lanes : Anti-COXIV Antibody at 1:2000 dilution
Lane 1: NIH/3T3 whole cell lysate
Lane 2: BA/F3 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: C6 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 20 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1
WB
Dilution image 1
1:2000
Legend image 2
Overlay histogram showing NIH/3T3 cells stained with AP22111a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22111a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 2
FC
Dilution image 2
1:25
Other Accession
P10888
Predicted
Rat
Isotype
Rabbit Ig
Calculated MW
19530
Antigen Source
HUMAN
Target/Specificity
This COXIV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 115-169 amino acids from the mouse region of human COXIV.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 200 ul
Available: In stock
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