RGMB Antibody

ArtNr	ABC-AP22293a-ev
Hersteller	Abcepta
Menge	50 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	RGMB, RGM domain family member B, DRG11-responsive axonal guidance and outgrowth of neurite, DRAGON
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Primary Accession	Q6NW40
Antigen Type	Recombinant Protein
Application	WB, IHC-P-Leica
Bio Background	Member of the repulsive guidance molecule (RGM) family that contributes to the patterning of the developing nervous system ( By similarity) . Acts as a bone morphogenetic protein (BMP) coreceptor that potentiates BMP signaling (By similarity). Promotes neuronal adhesion (By similarity). May inhibit neurite outgrowth.
Bio References	Ota T., et al.Nat. Genet. 36:40-45(2004). Schmutz J., et al.Nature 431:268-274(2004). Liu X., et al.Biochem. Biophys. Res. Commun. 382:795-800(2009). Bell C.H., et al.Science 341:77-80(2013).
Clonality	polyclonal
Clone/Animal Names	RB56812
Gene ID	285704
Gene Name	RGMB {ECO:0000303\|PubMed:19324014, ECO:0000312\|HGNC:HGNC:26896}
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-RGMB Antibody at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: Human brain lysate Lane 3: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	All lanes : Anti-RGMB Antibody at 1:2000 dilution Lane 1: U-251 MG whole cell lysate Lane 2: 293 whole cell lysate Lane 3: Human brain lysate Lane 4: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	Immunohistochemical analysis of paraffin-embedded human brain tissue using AP22293a performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 3	IHC-P-Leica
Dilution image 3	1:500
Isotype	Rabbit Ig
Calculated MW	47547
Antigen Source	HUMAN
Target/Specificity	This RGMB antibody is generated from a rabbit immunized with a recombinant protein of human RGMB.

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ABC-AP22293a-ev-datasheet.pdf

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