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VINC Antibody (N-term)

VINC Antibody (N-term)

ArtNr	ABC-AP7426a-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Vinculin, VCL, Metavinculin, MV
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Primary Accession	P18206
Antigen Type	Synthetic Peptide
Application	WB, IHC-P
Bio Background	VINC is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia.
Bio References	Moiseyeva E.P., Weller P.A.J. Biol. Chem. 268:4318-4325(1993) Sun N., Critchley D.R., Paulin D.Biochem. J. 409:657-667(2008) Izard T., Evans G., Borgon R.A.Nature 427:171-175(2004)
Clonality	Polyclonal
Clone/Animal Names	RB15469
Gene ID	7414
Gene Name	VCL
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M, Rat
Legend image 1	Western blot analysis of anti-VINC Antibody (N-term) (Cat.#AP7426a) in K562 cell line lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Western blot analysis of anti-VINC Antibody (N-term) (Cat.#AP7426a) in mouse lung tissue lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	All lanes : Anti-VINC Antibody (N-term) at 1:2000 dilution Lane 1: RD whole cell lysates Lane 2: Hela whole cell lysates Lane 3: rat liver lysates Lane 4: human testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 124 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:2000
Legend image 4	Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with VINC antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:10~50
Other Accession	P85972; P26234; Q64727; P12003
Predicted	C, Pig
Isotype	Rabbit Ig
Calculated MW	123799
Antigen Region	12-39
Antigen Source	HUMAN
Target/Specificity	This VINC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human VINC.
NCBI Accession	NP_003364.1; NP_054706.1

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