Vergleich

RHOXF2B Antibody (Center)

ArtNr ABC-AW5505-U100
Hersteller Abcepta
Menge 100 ul
Kategorie
Typ Antibody Primary
Applikationen WB, IF
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products RHOXF2B, Rhox homeobox family member 2B
Lieferbar
Primary Accession
P0C7M4
Antigen Type
Synthetic Peptide
Application
IF, WB
Bio Background
The function of this protein remains unknown.
Clonality
Polyclonal
Gene ID
727940
Gene Name
RHOXF2B (HGNC:33519)
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Fluorescent confocal image of A549 cell stained with RHOXF2B Antibody (Center)(Cat#AW5505).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RHOXF2B primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).RHOXF2B immunoreactivity is localized to Nucleus significantly.
Type image 1
IF
Dilution image 1
1:10~50
Legend image 2
All lanes : Anti-RHOXF2B Antibody (Center) at 1:1000 dilution
Lane 1: A549 whole cell lysates
Lane 2: K562 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 32 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2
WB
Dilution image 2
1:1000
Isotype
Rabbit Ig
Calculated MW
31637
Antigen Region
110-141
Antigen Source
HUMAN
Target/Specificity
This RHOXF2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-141 amino acids from the Central region of human RHOXF2B.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ul
Lieferbar: In stock
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