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GFAP (Astrocyte & Neural Stem Cell Marker) (GFAP/2076), CF640R conjugate, 0.1mg/mL

ArtNr B-BNC402076-100
Hersteller Biotium
Menge 100 uL
Quantity options 100 uL 500 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen FC, IHC
Clon GFAP/2076
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1
Konjugat/Tag CF640R
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Type
Primary
Manufacturer - Applications
Flow, intracellular (verified)|IHC, FFPE (verified)
Manufacturer - Category
Antibodies
Manufacturer - Targets
GFAP
Manufacturer - Conjugate / Tag
CF640R
Manufacturer - Host
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Shipping Temperature
Room temperature
Storage Conditions
Store at 2 to 8°C|Protect fluorescent conjugates from light
Molecular Weight
~50 kDa
Manufacturer - Research Area
Neuroscience
Description
This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Special handling for PI and label
Protect from light
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodology
immunostaining detection application
Classified or regulated chemicals
0.05% sodium azide (CAS 26628-22-8)
Concentration
0.1 mg/mL
Storage buffer
PBS, 0.1% BSA, 0.05% azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
Recombinant human GFAP protein fragment (around aa 101-200) (exact sequence is proprietary)
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
GFAP
Unigene
514227
Positive Control
Brain or Astrocytoma
Antibody target cellular localization
Cytoskeleton
Cell tissue expression
Glia
Validated in protein array
Monospecific
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Western blotting 1-2 ug/mL|Optimal dilution for a specific application should be determined by user
Manufacturer - Antibody Reactivity
GFAP

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 uL
Lieferbar: In stock
lieferbar

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