CD3e(RIV9), 0.2mg/mL

Primary

Antibodies

Purified, with BSA

Room temperature

20 kDa

Reacts with five invariable CD3 chains designated CD3y, CD3d, CD3e, CD3f, and CD3n with molecular weight ranging from 16-28 kDa.  CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.

Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Stable at room temperature or 37°C (98°F) for 7 days.

Primary and secondary antibodies for multiple methodology
immunostaining detection application

0.2 mg/mL

Guaranteed for at least 24 months from date of receipt when stored as recommended

Monoclonal

CD3E

Jurkat cells. Tonsil and lymph node

Lymphocytes|T-cells

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

CD3 epsilon | CD3 TCR complex | CD3E | CD3e antigen epsilon polypeptide (TiT3 complex) | Leu-4 epsilon chain | T cell antigen receptor complex epsilon subunit of T3 | T-cell surface antigen T3 | T-cell surface glycoprotein CD3 epsilon chain | T3E | TCRE | TiT3 complex