Hersteller |
IchorBio
|
Kategorie |
|
Typ |
Antibody |
Specific against |
Mouse |
Clon |
GK1.5 |
Isotype |
IgG2b Kappa |
Applikationen |
WB, Blocking |
Menge |
1mg |
Host |
Rat |
ArtNr |
ICH1042-1MG |
Targets |
CD4 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Aggregation |
Aggregation level <= 5% |
Antigen Distribution |
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines. |
Application Notes |
Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation. Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation. |
Concentration |
>= 2.0 mg/ml |
Endotoxin |
<= 1.0 EU/mg as determined by the LAL method |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Immunogen |
Mouse CTL clone V4 |
Product Type |
Low Endotoxin, anti-mouse |
Purification method |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
Storage |
anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8C. For long term storage aseptically aliquot in working volumes without diluting and store at -80C. Avoid Repeated Freeze Thaw Cycles. |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.