ArtNr |
ICH1042-50MG |
Hersteller |
IchorBio
|
Menge |
50 mg |
Quantity options |
100 mg
1 mg
25 mg
50 mg
5 mg
|
Kategorie |
|
Typ |
Antibody |
Applikationen |
WB, FC, IP, IHC, Blocking |
Clon |
GK1.5 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Rat |
Isotype |
IgG2b Kappa |
Purity |
>95% by SDS-PAGE and HPLC |
Citations |
Cao et al. Injectable hydrogel loaded with lysed OK-432 and doxorubicin for residual liver cancer after incomplete radiofrequency ablation. Journal of Nanobiotechnology |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3 |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Specificity |
Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4 |
Manufacturer - Applications |
Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation |
Manufacturer - Category |
Low Endotoxin, anti-mouse, monoclonal antibody |
Manufacturer - Isotype |
Rat IgG2b Kappa |
Manufacturer - Targets |
CD4 |
Shipping Temperature |
Blue ice |
Storage Conditions |
anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
Aggregation |
Aggregation level ≤ 5% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Antigen Distribution |
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines. |
Application Notes |
Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation. Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation. |
Concentration |
≥ 2.0 mg/ml |
Endotoxin |
≤ 1.0 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4 |
Purification method |
This monoclonal antibody was purified using Protein G |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Immunogen |
Mouse CTL clone V4 |
Related Antibodies |
|
Isotype Control |
Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243) |
Buffer |
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.