TRIM71 Antibody

TRIM71 antibody can be used for detection of TRIM71 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

TRIM71 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

TRIM71 Antibody: TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.

TRIM71 Antibody is affinity chromatography purified via peptide column.

PRS-10-301-4um

TRIM71 Antibody is supplied in PBS containing 0.02% sodium azide.

TRIM71 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TRIM71.

The immunogen is located within amino acids 680 - 730 of TRIM71.

tripartite motif-containing 71

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.