Anti-Sprouty antibody (C-Term) (STJ70571)

Pep-ELISA/WB/IF/FC/IHC

Store at-20°C on receipt and minimise freeze-thaw cycles.

0.5 mg/mL

Polyclonal

Peptide ELISA: antibody detection limit dilution 1:16000.
WB: Approx 37kDa band observed in Human Kidney lysates (calculated MW of 35.1kDa according to NP_005832.1 ). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells (PMID: 20068162). Recommended concentration: 1-3µg/ml.
IHC: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3µg/ml.
IF: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

C-Term