Recombinant Mouse IGF-1

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Mouse
Menge 10 ug
Host E.coli
ArtNr CS-CRI501A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Insulin-like Growth Factor I, IGF-I, is a growth factor produced in response to growth hormone-stimulated liver and can be found circulating throughout the body and throughout life. IGF-I activates the IGF-I receptor (IGF1R) and the insulin receptor to mediate growth of almost every cell of the body. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Mature human IGF-I is 100% homologous with bovine and pig proteins. Recombinant mouse IGF-I is a non-glycosylated protein, containing 70 amino acids, with a molecular weight of 7.6 kDa.
Formulation
Recombinant mouse IGF-1 is lyophilized with no additives.
Reconstitution
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Storage and Stability
Lyophilized product is very stable at -20C. Reconstituted material should be aliquoted and frozen at -20C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
GeneID
16000
Source
E. coli
Molecular Weight
7.6 kDa.
Purity
98% determined by: HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm
Specific Activity
1 x 106 U/mg
Endotoxin Level
Measured by kinetic LAL analysis and is typically <= 1 EU/ug protein.
Biological Activity
The activity is determined by the dose-dependent proliferation of FDC-P1 cells and is typically less than 20 ng/mL.
AA Sequence
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA

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Menge: 10 ug
Lieferbar: In stock
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