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Caki-2 growing culture

Caki-2 growing culture

ArtNr	CLS-330140
Hersteller	CLS Cell Lines Service
Menge	1 cell culture flask
Kategorie	Cell Lines & Cell Culture Cell Lines
Typ	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	CAKI-2, CaKi-2, caki-2, CAKI 2, Caki 2, Caki2, CAKI2
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Growth properties	Monolayer, adherent
Age	69 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like. Ultrastructural features include microvilli and microfilaments. Few mitochondria, lysosomes or lipid droplets. Frequent multilamellar bodies. No virus particles.
Biosafety Level	1
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	2 - 3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-ACF (CLS catalog number 800650)
Amelogenin	X, Y
Tumorigenic	Yes, in nude mice; forms clear cell carcinoma
Split Ratio	A ratio of 1:3 to 1:6 is recommended
Seeding Density	1 x 10^4 cells/cm^2 will result in a 90% confluent monolayer in about 4 days
Karyotype	(P8) hypopentaploid to hypohexaploid (+A2, +A3, +B, +C, +D, +F, +G, -A) with abnormalities including dicentrics, acrocentric fragments, minutes, breaks, and large subtelocentric markers
Isoenzymes	Me-2, 1; PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0511
Manufacturer - Citation	Caki-2 (CLS catalog number 300140)
CSF1PO	10, 12
D13S317	10
D16S539	9, 13
D5S818	11
D7S820	12
TH01	6
TPOX	9, 11
vWA	16, 17
D3S1358	14
D21S11	27, 31
D18S51	17
pentaE	7, 17
pentaD	10, 13
D8S1179	10
FGA	22

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 1 cell culture flask

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Lieferung vsl. bis 28.08.2025

Express-Lieferung: 3 Tage sparen

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