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Caki-2 growing culture

Caki-2 growing culture

Item no.	CLS-330140
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	CAKI-2, CaKi-2, caki-2, CAKI 2, Caki 2, Caki2, CAKI2
Available
Growth properties	Monolayer, adherent
Age	69 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like. Ultrastructural features include microvilli and microfilaments. Few mitochondria, lysosomes or lipid droplets. Frequent multilamellar bodies. No virus particles.
Biosafety Level	1
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	2 - 3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-ACF (CLS catalog number 800650)
Amelogenin	X, Y
Tumorigenic	Yes, in nude mice; forms clear cell carcinoma
Split Ratio	A ratio of 1:3 to 1:6 is recommended
Seeding Density	1 x 10^4 cells/cm^2 will result in a 90% confluent monolayer in about 4 days
Karyotype	(P8) hypopentaploid to hypohexaploid (+A2, +A3, +B, +C, +D, +F, +G, -A) with abnormalities including dicentrics, acrocentric fragments, minutes, breaks, and large subtelocentric markers
Isoenzymes	Me-2, 1; PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0511
Manufacturer - Citation	Caki-2 (CLS catalog number 300140)
CSF1PO	10, 12
D13S317	10
D16S539	9, 13
D5S818	11
D7S820	12
TH01	6
TPOX	9, 11
vWA	16, 17
D3S1358	14
D21S11	27, 31
D18S51	17
pentaE	7, 17
pentaD	10, 13
D8S1179	10
FGA	22

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 8/28/2025

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