Phospho-NFKBIA (Ser32/Ser36) Antibody

NFKBIA

I kappa B alpha antibody; I-kappa-B-alpha antibody; IkappaBalpha antibody; IkB-alpha antibody; IKBA antibody; IKBA_HUMAN antibody; IKBalpha antibody; MAD 3 antibody; MAD3 antibody; Major histocompatibility complex enhancer-binding protein MAD3 antibody; NF kappa B inhibitor alpha antibody; NF-kappa-B inhibitor alpha antibody; NFKBI antibody; NFKBIA antibody; Nuclear factor of kappa light chain gene enhancer in B cells antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody

Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a.

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823.
Honda, R. et al. (1997) FEBS Lett. 420, 25-27.
Hirao, A. et al. (2000) Science 287, 1824-1827.
Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157.
Chehab, N.H.et al. (1999) Proc .Natl .Acad. Sci. USA 96, 13777-13782.

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugateds. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Signal Transduction

Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a.